Blood Groups Practical Theory Quiz

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1. If a patient has a positive antibody screen, a request for a red blood cell (RBC) product transfusion will usually be delayed due to the extra testing that is now required to identify the antibody and find compatible RBCs. Which of the following antibodies would be most likely to cause the SHORTEST transfusion delay?
2. When a patient makes an immune antibody, red blood cell products are phenotyped and products that are antigen-negative for the corresponding antibody selected for transfusion. RBC products are already pre-phenotyped to prevent interactions with one immune antibody. Which antibody is it?
3. A patient has anti-c. If 80% of donors are c-positive and 68% are C-positive, how many RBC units will the transfusion service need to test in order to find 2 units that are compatible with the patient?
4. If a patient has Anti-c and Anti-S, how many RBC units will the transfusion service need to test in order to find 2 units that are compatible with the patient?
(Frequency data: c = 80%, C = 68%, s = 90%, S = 55%)
5. A patient has a positive antibody screen and positive results against cells in the antibody panel. The patient specimen is retested with antibody panel cells that have been treated with the enzyme "ficin." The antibody no longer reacts against cells in the antibody panel. Which of the following antibodies is most consistent with these results?
6. A patient has a positive antibody screen and a positive antibody panel. All tested cells are positive (3+) at the AHG phase of reactivity. The only tested cell that is negative is the autocontrol. You phenotype the patient and discover that he is negative for the following antigens: E, Fy(a), S, and Jk(b). You locate a testing cell that has this antigen negative phenotype. The cell reacts 3+ at the AHG phase. Of the following choices, which is the most likely specificity of the antibody/antibodies?
7. A patient has a positive antibody screen and a positive antibody identification panel. All tested cells are positive (3+) at the AHG phase of reactivity. The autocontrol is also positive (3+) at the AHG phase. The patient has not been transfused or pregnant in the preceding 6 months. Which technique would you use to complete the case?
8. A patient with sickle cell disease who was transfused 2 units of red blood cells 2 weeks ago at another facility presents to your transfusion service for the first time. The clinician is requesting phenotypically matched red cells. What serologic technique can be used to obtain the patient phenotype in this case?
9. Your lab performs tube testing with LISS potentiation for routine antibody screening and identification. You identify an RBC alloantibody in a patient using your usual techniques. Which antibody would be eligible for antibody screens and crossmatches using the "prewarm technique"?
10. Which of the following antibodies could be identified using a patient specimen collected in a red top tube (plain glass), but may not be identified if a purple top tube (EDTA anticoagulant) was used for the collection?

 

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