Quiz: The ABO Blood Group - Blood Bank Guy Quizzes

1. The reactivity of blood group A is confirmed by detecting the presence of which immunodominant sugar molecule?

N-acetyl-D-neuraminic acid

L-fucose

N-acetyl-D-galactosamine

N-acetyl-D-glucosamine

D-galactose

2. The mating of parents of which two ABO phenotypes can potentially produce offspring with ALL of the common four blood types?

AB and O

AB and A

AB and B

AB and AB

A and B

3. Bombay phenotype (O_h) individuals may have antibodies with all the following specificities EXCEPT:

Anti-A

Anti-B

Anti-H

Anti-O

Anti-A,B

4. Which cells agglutinate most strongly with Ulex europaeus lectin?

O and A2

A1 and A2

O and A1B

B and A2B

A1 and B

5. Immune A and B alloantibodies differ from non-red cell stimulated (naturally occurring) A and B alloantibodies in that the immune antibodies:

Are generally IgG rather than IgM

Are unable to cross the placenta

Can be enhanced in reactivity by incubation at 4^oC

Cause direct agglutination at room temperature

Rarely cause clinical hemolysis

6. Which ABH substances would you expect to find in the saliva of a group A secretor?

H only

H and A

H and B

H and O

A only

7. Which of the following is the best explanation for why the ABO system is the most important blood group system in transfusion safety?

It is the only system in which antibodies are normally produced for the antigens an individual lacks

ABO antibodies are capable of causing rapid, severe intravascular hemolysis

Reactions with ABO antibodies are the most common cause of transfusion-related death

ABO antibodies are often implicated in severe hemolytic disease of the fetus and newborn

Routine ABO forward and reverse grouping is difficult to interpret and fraught with error

8. A 26 year old pregnant female is being tested prior to a scheduled C-section tomorrow. Her cell grouping (forward typing) is consistent with blood group O, while her serum grouping (reverse grouping or "back-typing") appears to be group A. The most common reason for this type of ABO discrepancy is:

She has the Bombay phenotype

She is a non-secretor, so her plasma lacks A

Clerical errors or a sample mix-up

Use of an uncalibrated centrifuge

She has undiagnosed acute leukemia (AML)

9. An ABO discrepancy between forward and reverse grouping owing to weak-reacting or missing antibodies could be BEST explained by which of the following:

Patient has a subgroup of blood group A

Patient is very old or very young

Patient has acquired B phenotype

Patient has antibodies to low incidence antigens

Patient has antibodies against reagent preservatives

10. A blood donor has the genotype hh, AB. What is his apparent red cell phenotype during routine forward and reverse group typing?

Cannot be determined

11. Approximately what percentage of group A individuals could be further classified as subgroup A₁?

20%

40%

60%

80%

99%

12. Which of the following statements is TRUE regarding the A₂ blood group?

Dolichos biflorus lectin agglutinates A₂ but not A₁ RBCs

Ulex europaeus lectin will give stronger reactions with A₁ than with A₂ RBCs

A₂ RBCs have more H antigen than A₁ RBCs

If anti-A₁ is made by an A₂ person, it is usually clinically significant

Most A₂ individuals have a different form of anti-B than A₁ individuals

13. Which of the following genes codes for production of the same basic antigen as the gene known as H?

14. Alert! Completely absurd and unrealistic question coming! The labels have come off of some of the reagent bottles in your transfusion service, so the ABO testing reagents are just sitting there, label-less (and they just look ridiculous!). A new lab scientist is trying to find the anti-B, and he asks, "What color is anti-B?" You reply:

"Green"

"Orange"

"Blue"

"Yellow"

"Your mother was a hamster and your father smelt of elderberries!"

15. Which of the following statements is TRUE regarding Hemolytic Disease of the Fetus/Newborn (HDFN) caused by ABO antibodies?

Fetal hemolysis is typically severe

It rarely occurs during the first pregnancy

It is most common with O mothers and A babies

A negative cord blood direct antiglobulin test excludes it

It occurs less commonly than Rh HDFN

ABO Blood Group System Quiz

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